ANTIBACTERIAL ACTIVITY OF DIFFERENT ETHANOLIC EXTRACT OF ALGERIAN PROPOLIS AGAINST Staphylococcus aureus
ABDELLATIF BOUDRA *
Department of Animal Health, The Veterinary Institute, University of Tiaret, 14000, Algeria.
IDIR BENBELKACEM
Department of Animal Health, The Veterinary Institute, University of Tiaret, 14000, Algeria.
MOHAMED AMINE AISSA
Laboratory of Microbiology II, The Veterinary Institute, University of Tiaret, 14000, Algeria.
*Author to whom correspondence should be addressed.
Abstract
The antimicrobial activity of propolis extracts is well documented. Nevertheless, the properties of the Algerian propolis are poorly investigated. This fact could be due to the diversity of the climate and the geographic areas from which propolis is usually harvested. The present work is a contribution to evaluate the antimicrobial susceptibility testing of propolis varieties with high total polyphenols and flavonoids contents, chosen beforehand from an analysis made of 10 propolis samples. The disk diffusion method was employed to determine the antistaphylococcal activity of three EEAP (ethanolic extract of Algerian propolis) samples, at different concentrations (5%, 10, 20 and 30%), against a reference bacterium strain of Staphylococcus aureus, the most redoubtable agent in osteomyelitis. Inhibitory zone diameters for the tested bacterium were 14.99 mm for EEAP at 5% concentration, 16.41 mm at 10%, 18.08 mm at 20% and 19.16 mm at 30%, respectively. Our in vitro study revealed that EEAP with high polyphenols and flavonoids contents exhibit a strong antibacterial activity, and it may be indicated as an alternative solution for the treatment of staphylococcal osteomyelitis.
Keywords: Algerian propolis, Staphylococcus aureus, osteomyelitis, inhibition zone.