SEROLOGICAL AND MOLECULAR DIAGNOSIS OF Toxoplasma gondii
KHALED ABDELWAHED
Parasitology-Mycology Laboratory, Hospital Center of Ain Naâdja (HCA), Algiers, Algeria.
NORA MIMOUNE *
High National Veterinary School (HNVS), Bab-Ezzouar, Algiers, Algeria and Institute of Veterinary Sciences, LBRA, Saad Dahleb University, Blida 1, Algeria.
SMAHI NORA
Faculty of Nature and Life Sciences, Saad Dahleb University, Blida1, Algeria.
HAMOUDI ADJMI HAIET
Parasitology-Mycology Laboratory, Hospital Center of Ain Naâdja (HCA), Algiers, Algeria.
BEKHOUCHE SALIM
Parasitology-Mycology Laboratory, Hospital Center of Ain Naâdja (HCA), Algiers, Algeria.
BOUBEKEUR RACHA
Parasitology-Mycology Laboratory, Hospital Center of Ain Naâdja (HCA), Algiers, Algeria. 2High National Veterinary School (HNVS), Bab-Ezzouar, Algiers, Algeria.
BAAZIZI RATIBA
High National Veterinary School (HNVS), Bab-Ezzouar, Algiers, Algeria.
SAIDI RADHWANE
Department of Agronomy, University of Amar Telidji-Laghouat, Algeria.
BENAISSA MOHAMED HOCINE
Scientific and Technical Research Center for Arid Areas, Biophysical Station, Touggourt, Algeria.
KAIDI RACHID
Institute of Veterinary Sciences, LBRA, Saad Dahleb University, Blida 1, Algeria.
*Author to whom correspondence should be addressed.
Abstract
This study aimed to determine the immune status of pregnant women at the Central Hospital of Ain Naâdja (HCA) in Algeria. For this purpose, the identification of anti-Toxoplasma immunoglobulins IgG and IgM by ELFA test was carried out in 393 patients, as well as the comparison of the serological profile for the mother-child, serum-aqueous humor pairs by the Western Blot (WB) assay. Parasite search was done by standard PCR on placenta samples using JW62/JW63 primer. The results obtained from 393 serums collected showed that: 40.96% of the pregnant women having a serological profile in favor of an old Toxoplasmosis, and 59.03% were not immunized (P<0.05). Serological comparison by WB analysis of the pairs revealed that 90.1% of the couples possessing the same serological profile (negative), and 9.09% were positive (congenital toxoplasmosis) (P<0.05). To conclude, The PCR performed on placentas showed a high level of specificity for diagnosis. It allows early diagnosis and should be recommended for identification of congenital infection.
Keywords: Toxoplasma gondii, ELFA, western blolt, PCR, pregnant woman.