BIONATURE

BIONATURE, ISSN No. : 0970-9835 (Print), 0974-4282 (Online), Vol.: 38, Issue.: 2

Original Research Article

EFFECT OF KOREAN WINE WASTE EXTRACTION ON NITRIC OXIDE AND CYTOKINE PRODUCTION IN LIPOPOLYSACCHARIDE-INDUCED INFLAMMATION

 

JAE YUL BAEK1, MYOUNGWON CHOI2, INDAL PARK2 AND SUN YOUNG LIM1*

1The Division of Marine Bioscience, Korea Maritime and Ocean University, Busan, Korea.

2Department of Microbiology, Kosin University College of Medicine, Busan, Korea.

Abstracts

The effect of extract of Korean wine waste on the production of nitric oxide (NO) and cytokines in splenocytes and RAW 264.7 cells was investigated using lipopolysaccharide (LPS) as a stimulant. Treatment of RAW 264.7 cells with the 85% aqueous methanol (MeOH) fraction from Korean wine waste reduced LPS-induced NO production in a dose-dependent manner (p<0.05). Culture supernatants of splenocytes exposed to a co-treatment of 85% aq. MeOH fraction and LPS were harvested to determine the production levels of cytokines [IL-2, IL-4, IL-5, IL-6, IL-12/IL-23(p40) and IFN-γ]. As measured by ELISA, co-administration of 85% aq. MeOH fraction (10 μg/mL) with LPS significantly reduced the IL-2 expression after 48 h of treatment, as compared to treatment with only LPS (p<0.05), where the level of IL-6 expression was significantly lower after 24 h of the co-treatment (p<0.05). The IL-12/IL-23(p40) expression levels after 24 and 72 h of co-treatment were also significantly lower than that from LPS treatment only (p<0.05). However, the IL-4 and IFN-γ expression levels were significantly lower only after 72 h of co-treatment. These results indicate that the 85% aq. MeOH fraction from Korean wine waste decreased the production of NO as well as the expression of the pro-inflammatory cytokines IL-2, IL-6 and IL-12/IL-23(p40).

Keywords :

Korean wine waste; nitric oxide; interleukin-2; interleukin-6; interleukin-IL-12/IL-23(p40).

Announcement

Founding Chief Editor:

Prof. Hit Kishore Goswami Ph.D., Retired & Visiting Professor of Botany and Genetics, University of Bhopal, Address: 24, Kaushalnagar, P O Misrod, Bhopal (MP) India


Disclaimer: BIONATURE (0970-9835 (Print), 0974-4282 (Online)) journal is published by Global Press Hub (GPH) from 2017 (Volume 37). BIONATURE (0970-9835 (Print), 0974-4282 (Online)) is the official journal of Society of Bionaturalists (India). BIONATURE (0970-9835 (Print), 0974-4282 (Online)) is registered with Registrar of Newspapers, India, No: 9680, dated 11.12.1980.

 

Society Address: Society of Bionaturalists, Department of Genetics, Bhopal University (New name: Barkatullah University), Bhopal, Madhya Pradesh 462026, India. (India), website: www.bionaturalists.in

Chairman: Prof. Hit Kishore Goswami Ph.D., Retired & Visiting Professor of Botany and Genetics, University of Bhopal, Address: 24, Kaushalnagar, P O Misrod, Bhopal (MP) India